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1H-NMR spectra of purified cyclic β-1,2-glucans from B. abortus. (A) B. abortus S19. (B) B. abortus S19 treated with alkali. (C) B. abortus Cgm19. The two signals between 2.4 and 2.6 ppm correspond to the methylene protons of the succinic acid substituents. In panel A, the resonance at 3.16 is derived from methanol. The resonance at 4.7 is H2O. Chemical shifts are expressed relative to external 1,4-dioxane.
