TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Phenotype or genotypea | Reference or source |
|---|---|---|
| Strains | ||
| E. coli XL1-blue MRF | Δ(mcrA)183 Δ(mcrB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac[F′ proAB lacIqZΔM15 Tn10(Tetr)] | 26 |
| E. coli S17.1(λpir) | λ lysogenic S17.1 derivative producing π protein for replication of plasmids carrying oriR6K (Nals) | K. N. Timmis |
| B. abortus | ||
| 2308 | Virulent; field isolated; wild type; Nalr erythritolr | 38 |
| Cgm08 | B. abortus 2308 cgm mutant; cgm::Kmr | This study |
| Cgm08(pBB4cgm) | B. abortus 2308 cgm mutant with plasmid pBB4cgm; Kmr Ampr | This study |
| B. abortus | ||
| S19 | Vaccine strain; Nalr erythritols; naturally occcuring derivative of B. abortus 2308 | 38 |
| Cgm19 | B. abortus S19 cgm mutant; cgm::Kmr | This study |
| Cgm19(pBB4cgm) | B. abortus S19 cgm mutant with plasmid pBB4cgm; Kmr Ampr | This study |
| Plasmids | ||
| pBBR1MCS-4 | Broad-host-range cloning vector (Ampr) | 28 |
| pBB4cgm | pBBR1MCS-4 containing B. abortus cgm gene; Ampr | This study |
| pvTcgm | T-easy vector containing B. abortus cgm gene; Ampr | This study |
a
Ampr, ampicillin resistance: Cbr, carbenicillin resistance: Kmr, kanamycin resistance, Nalr, nalidixic acid resistance.