TABLE 3.

Osmolarity affects the substitution of B. abortus cyclic β-1,2-glucan^a

Solute (mM)	Osmolarity (mmol/kg)b	Total glucansc	Neutral glucansd	Anionic glucanse	Anionic/neutral glucans
0 NaCl	109	19.05	29.28	70.72	2.42
170 NaCl	391	20.23	48.13	51.87	1.08
170 KCl	387	19.41	48.21	51.79	1.07
110 K2SO4	338	19.20	48.32	51.68	1.07
340 sucrose	441	20.15	49.01	50.99	1.04

^aB. abortus strain 2308 was cultured in 100 ml of LB or LB with the addition of 170 mM NaCl, 170 mM KCl, 110 mM K₂SO₄, and 340 mM sucrose at 37°C for 48 h. The cell pellets were extracted with 70% ethanol, and the neutral- and anionic-cyclic-β-1,2-glucan contents were determined after Bio-Gel P4 chromatography and DEAE-Sephadex chromatography as described in Materials and Methods.

^bThe osmolarity of the medium was measured on a Vapro-Vapor Pressure Osmometer (model 5520; WESCOR).

^cThe total cellular-glucan content is expressed as milligrams of glucose equivalent per liter of culture.

^dThe neutral-glucan content is expressed as a percentage of the total cellular-glucan content.

^eThe anionic-glucan content is expressed as a percentage of the total cellular-glucan content.