TABLE 1.
Strains
| Straina | Description | Source or reference |
|---|---|---|
| S. enterica strains | ||
| TE6134 | hfq-1::Mud-Cam | 6 |
| TE6153 | putPA1303::Kanr-katE-lac [op] | 6 |
| TE6266 | hfq-1::Mud-Cam putPA1303::Kanr-rpoS-lac [pr] | 7 |
| TE6369-2 | hfq-1::Mud-Cam putPA1303::Kanr-rpoS-lac [pr] (C470G) | 7 |
| TE6369-3 | hfq-1::Mud-Cam putPA1303::Kanr-rpoS-lac [pr] (G550C) | 7 |
| TE6382 | hfq-1::Mud-Cam putPA1303::Kanr-rpoS-lac [pr] (C470G) (G550C) | 7 |
| TE6557 | hfq-1::Mud-Cam putPA1303::Kanr-rpoS-lac [pr] (C469G) | 7 |
| TE6558 | hfq-1::Mud-Cam putPA1303::Kanr-rpoS-lac [pr] (G551C) | 7 |
| TE6590 | hfq-1::Mud-Cam putPA1303::Kanr-rpoS-lac [pr] (C469G) (G551C) | 7 |
| TE6850 | clpX1::Tn10d-Cam | 10 |
| TE6851 | mviA22::Tn10d-Cam | 10 |
| TE8007 | JF3490 dksA4::Tn10d-Tet | J. Foster |
| TE8012 | TE8007 LT2A putPA1303::Kanr-katE-lac [op] | |
| TE8544 | putPA::katE-lac [op] rpoS1074::tetAR (A.S., deletes nt 461-464 of rpoS leader) | |
| TE8546 | putPA::katE-lac [op] rpoS1076::cat (deletes from bp 110 upstream to 469 of rpoS leader) | |
| TE8566 | putPA::katE-lac [op] ΔdsrA::tetAR | |
| TE8567 | putPA::katE-lac [op] ΔrprA::tetAR | |
| TE8587 | putPA::katE-lac [op] ΔdsrA::cat | |
| TE8588 | putPA::katE-lac [op] ΔcysC::tetAR | |
| TE8589 | putPA::katE-lac [op] rpoS1084::tetAR (AGGA, deletes nt 554-557 of rpoS leader) | |
| TE8607 | ΔcysC::tetAR | |
| TE8608 | putPA1303::Kanr-katE-lac [op] ΔdsrA::cam | |
| TE8610 | putPA1303::Kanr-katE-lac [op] ΔrprA::tetAR | |
| TE8613 | putPA1303::Kanr-katE-lac [op] ΔdsrA::cam ΔrprA::tetAR | |
| TE8622 | ΔcysC::tetAR rpoS1076::cat (deletes from bp 110 upstream to 469 of rpoS leader) | |
| TE8701 | putPA::katE-lac [op] rpoS1080::tetAR (ΔA.S.-AGGA, deletes nt 461-557) | |
| TE8737 | rpoS1082::MudJ (codon 66) | |
| TE8794 | rpoS1083::MudJ (codon 222) | |
| TE8804 | nlpD::MudJ (nlpD codon 213, nt 9 of rpoS leader) | |
| TE8805 | rpoS1079::MudK (codon 187) | |
| TE8807 | rpoS1077::MudK (codon 22) | |
| TE8808 | rpoS1078::MudK (codon 216) | |
| TE8810 | rpoS1081::MudK (codon 250) | |
| TE8815 | rpoS1078::MudK (codon 216) hfq-1::Mud-Cam | |
| TE8852 | TE8815 rpoS (C469G) | |
| TE8853 | TE8815 rpoS (C470G) | |
| TE8854 | TE8815 rpoS (G471C) | |
| TE8855 | TE8815 rpoS (C549G) | |
| TE8856 | TE8815 rpoS (G550C) | |
| TE8857 | TE8815 rpoS (G551C) | |
| TE8858 | TE8815 rpoS (C469G) (G551C) | |
| TE8859 | TE8815 rpoS (C470G) (G550C) | |
| TE8860 | TE8815 rpoS (G471C) (C549G) | |
| TE8935 | nlpD::MudJ (nlpD codon 302, nt 276 of rpoS leader) | |
| TE8936 | rpoS:::MudJ (codon 36) | |
| TE8983 | rpoS1077::MudK (codon 22) ΔdsrA::cat | |
| TE9049 | nlpD::[FRT-lacZY pKG137 Kanr] (nlpD codon 302, nt 276 of rpoS leader) | |
| TE9050 | rpoS::[FRT-lacZY pKG137 Kanr] (rpoS codon 36) | |
| TE9051 | rpoS::[FRT-lacZY pKG137 Kanr] (rpoS codon 222) | |
| TE9052 | nlpD::[FRT-lacZY pKG137 Kanr] (nlpD codon 213, nt 9 of rpoS leader) | |
| TE9053 | rpoS::[FRT-lacZY pKG137 Kanr] (rpoS codon 4) | |
| TE9160 | rpoS1081::MudK (codon 250) (TTG→ATG start codon) | |
| TE9179 | MS1868 StrA1 rpoS::[tetAR rpsL+] (AGGA) | S. Maloy |
| TE9213 | rpoS1081::MudK (codon 250) (TTG→ATG start codon) ΔdsrA::cat | |
| TE9219 | rpoS1081::MudK (codon 250) (TTG→ATG start codon) ΔdsrA::cat ΔrprA::tetAR | |
| TE9236 | TE8815 rpoS (G461C) | |
| TE9237 | TE8815 rpoS (G461A) | |
| TE9238 | TE8815 rpoS (G461T) | |
| TE9239 | TE8815 rpoS (G462C) | |
| TE9240 | TE8815 rpoS (G462A) | |
| TE9241 | TE8815 rpoS (G462T) | |
| TE9242 | TE8815 rpoS (G463C) | |
| TE9243 | TE8815 rpoS (G463A) | |
| TE9244 | TE8815 rpoS (G463T) | |
| TE9245 | TE8815 rpoS (G464C) | |
| TE9246 | TE8815 rpoS (G464A) | |
| TE9247 | TE8815 rpoS (G464T) | |
| TE9316 | rpoS1077::MudK (codon 22) zef-6829::tetAR rcsC55 (T903A) | |
| TE9317 | rpoS1077::MudK (codon 22) zef-6829::tetAR rcsC+ | |
| TE9318 | rpoS1077::MudK (codon 22) zef-6829::tetAR rcsC64 (F473I) | |
| TE9333 | gmm-21::MudJ zef-6829::tetAR rcsC55 (T903A) | |
| TE9334 | gmm-21::MudJ zef-6829::tetAR rcsC+ | |
| TE9352 | rpoS1077::MudK (codon 22) zef-6829::tetAR rcsC55 (T903A) ΔrprA::cam | |
| TE9353 | rpoS1077::MudK (codon 22) zef-6829::tetAR rcsC+ ?rprA::cam | |
| TE9354 | rpoS1077::MudK (codon 22) zef-6829::tetAR rcsC64 (F473I) ΔrprA::cam | |
| TE9368 | gmm-21::MudJ zef-6829::tetAR rcsC64 (F473I) | |
| TE9394 | gmm-21::MudJ zef-6829::tetAR rcsC+ ?rprA::cam | |
| TE9395 | gmm-21::MudJ zef-6829::tetAR rcsC55 (T903A) ΔrprA::cam | |
| TE9396 | gmm-21::MudJ zef-6829::tetAR rcsC64 (F473I) ΔrprA::cam | . |
| TE9426 | TE8807/pNM12 (vector) | |
| TE9427 | TE8983/pNM12 (vector) | |
| TE9428 | TE8983/pNM3 (PBAD-E. coli dsrA+) | |
| TE9429 | TE8807/pBAD18 (vector) | |
| TE9430 | TE8983/pBAD18 (vector) | |
| TE9431 | TE8983/pTE780 (PBAD-S. enterica dsrA+) | |
| TE9452 | TE8815 rpoS (T468C) | |
| TE9453 | TE8815 rpoS (C476A) (C477A) | |
| E. coli strains | ||
| SG22182 | MC4100 ara mal::lacIQ | D. Sledjeski |
| DDS1365 | MC4100 ara mal::lacIQdsrA1::cat | D. Sledjeski |
| TE6897 | MC4100 ara mal::lacIQtrpDC700:: putPA1303::Kanr-katE-lac [op] | |
| TE6898 | MC4100 ara mal::lacIQtrpDC700:: putPA1303::Kanr-rpoS-lac [pr] | |
| TE6913 | MC4100 ara mal::lacIQtrpDC700:: putPA1303::Kanr-katE-lac [op] dsrA1::cat | |
| TE6914 | MC4100 ara mal::lacIQtrpDC700:: putPA1303::Kanr-rpoS-lac [pr] dsrA1::cat | |
| TE8096 | BW26678 E. coli K-12/pKD46 [AmpR, pSC101 rep (Ts) araC+ PBAD-λ red] | B. Wanner |
| TE9062 | zgd::tetAR (just upstream of rpsL+) | |
| TE9416 | TE6898/pBAD18 (vector) | |
| TE9418 | TE6914/pBAD18 (vector) | |
| TE9419 | TE6914/pTE780 (PBAD-S. enterica dsrA+) | |
| TE9422 | TE6898/pNM12 (vector) | D. Sledjeski |
| TE9424 | TE6914/pNM12 (vector) | |
| TE9425 | TE6914/pNM3 (PBAD-E. coli dsrA+) | D. Sledjeski |
a
Numbering corresponds to the position of the last nucleotide retained from the rpoS leader or the codon within which the fusion occurs (i.e., the last intact codon is one previous).