TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Parent strain or plasmid | Reference or construction details |
|---|---|---|---|
| Strains | |||
| EMG2 | (Wild type) K-12 F+ λ+ | 1 | |
| KLE416 | ΔglpD::FRT | EMG2 | This study |
| E4152 | EcoK r− m− McrBC− Mrr− Dam+ Dcm+ carrying pACYC184 | Er2420 | NEB |
| JM109 | e14− (McrA−) recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 Δ(lac-proAB) (F′ traD36 proAB lacIqZΔM15) | Stratagene | |
| HM22 | zde-264::Tn10 dapA6 hipA7 | HM21 | 39 |
| KL312 | ΔhipBA::FRT | EMG2 | 31 |
| KLE401 | JM109 carrying pACYC184 | JM109 | This study |
| KLE414 | EMG2 carrying pBADglpD | EMG2 | This study |
| KLE413 | ΔhipBA::FRT ΔglpD::kan | KL312 | This study |
| KLE418 | ΔglpABC::kan | EMG2 | This study |
| KLE421 | EMG2 carrying pBADcontrol | EMG2 | This study |
| KLE443 | EMG2 carrying pBAD3-2 | EMG2 | This study |
| KLE4481 | ΔmgsA::FRT | EMG2 | This study |
| KLE449 | ΔgloA::kan | EMG2 | This study |
| MV1703 | ΔglpR::FRT | EMG2 | This study |
| MV1723 | ΔglpR::FRT ΔglpD::kan | MV1703 | P1 from KOJW3389 |
| CAG12164 | malF3089::Tn10 | C. Gross | C. Gross |
| KLE456 | ΔdgkA::kan malF3089::Tn10 | KOJW4002 | P1 from CAG1216 |
| BB26-36 | plsB26 plsX50 glpD3 glpR2 phoA8 StrsrelA1 spoT1 tonA22 T2R pit-10 HfrC | Strain 8 | 22 |
| KLE459 | BB26-36 malF3089::Tn10 | BB26-36 | P1 from KLE456 |
| KLE456 | malF3089::Tn10 | EMG2 | P1 from KLE459 |
| KLE457 | malF3089::Tn10 plsB26 | EMG2 | P1 from KLE459 |
| TJS2 | malT::Tn10 glpR12 | T. J. Larson | |
| MV1714 | malT::Tn10 | EMG2 | P1 from TJS2 |
| MV1715 | malT::Tn10 glpR12 | EMG2 | P1 from TJS2 |
| MV1718 | malT::Tn10 ΔglpD::kan | MV1714 | P1 from KLE416 |
| MV1719 | malT::Tn10 glpR12 ΔglpD::kan | MV1715 | P1 from KLE416 |
| KLE458 | ΔglpQ::kan | EMG2 | P1 from KOJW2233 |
| KOJW3414 | ΔugpQ::kan | BW25113 | 4 |
| KLE459 | ΔugpQ::kan | EMG2 | P1 from KOJW3414 |
| KOJW4323 | ΔmdoB::kan | BW25113 | 4 |
| KLE460 | ΔmdoB::kan | EMG2 | P1 from KOJW4323 |
| KOJW1037 | ΔmdoH::kan | BW25113 | 4 |
| KLE461 | ΔmdoH::kan | EMG2 | P1 from KOJW1037 |
| KOJW3897 | ΔglpK::kan | BW25113 | 4 |
| KLE462 | ΔglpK::kan | EMG2 | This study |
| KLE463 | ΔglpD::FRT ΔglpK::kan | KLE416 | P1 from KOJW3897 |
| Plasmids | |||
| pKD13 | Template plasmid aphA | 19 | |
| pKD46 | λ Red recombinase expression plasmid bla | 19 | |
| pACYC184 | p15A cat tet | NEB | |
| pBAD33 | P15 cat ara pBAD | 14 | |
| pBAD3035 | P15 cat ara pBAD::relE | pBAD33 | 13 |
| pBADglpD | relE removed from MCS with SphI and XbaI and replaced with glpD in those same sites | pBAD3035 | This study |
| pBAD3-2 | Site-directed mutant, Gly15Arg | pBADglpD | This study |