FIG. 3.

A pleD mutant shows reduced surface binding and delayed holdfast formation. SW cells of CB15 wild type (ATCC 19089) (open squares) and an isogenic ΔpleD mutant (open circles) were purified and suspended in fresh PYE medium. Aliquots were removed from the synchronized culture throughout the cell cycle at 15-minute intervals, transferred to microtiter plates, and allowed to attach to the plastic surface for 15 min. Attachment (open symbols) and holdfast formation (closed symbols) were quantified as indicated in Fig. 2. Cell cycle progression is indicated as cell cycle units. The error bars indicate standard deviations.