FIG. 3.

Time course of minus-strand (left panel) and plus-strand (right panel) RNA accumulation in protoplasts transfected with RNA3 containing a wild-type AUA triloop or mutant AUG (mutation underlined) or GUA triloops. Transfected cells were lysed, and RNA was extracted at the times indicated on the horizontal axes. Where there was a mutation in RNA3, the mutated nucleotide is underlined in the name of the RNA. The results of a Northern blot were quantified using a PhosphorImager and expressed in phosphorimage (PI) units.