FIG. 4.

Effect of mutations in the triloop stem and the closing base pair on BMV RNA3 replication. (The names of mutated nucleotides are underlined.) (A) Schematic of SLC, with the insertion, deletion, and point mutations shown in boxes. Only the affected regions of RNA3 are shown. The names of the mutant RNAs are in parentheses. (B) Autoradiogram showing effects of mutations on genomic minus- and plus-strand (as indicated to the right of the autoradiograms) accumulation. Total RNA was extracted from transfected protoplasts, and Northern analysis was carried out using probes specific for plus-strand, minus-strand, and 18S RNAs. The names of the mutant RNA3s used in the transfections are shown above the autoradiograms, and the identities of the RNAs in each image are indicated to the left of the autoradiograms.