TABLE 1.

Effect of mutations in SLC on RNA synthesis in vitro and in protoplasts

Nucleotide change or RNA3 derivative	Percent RNA synthesis in vitroa	Negative-strand synthesis ratio (RNA3/RNA2)	Positive-strand synthesis ratio (RNA3/RNA2)
Triloop
AUA (wt)b	100	9.0	4.3
AUGc	132 ± 7	8.5	3.7
GUA	8 ± 1	0.5	0.8
Triloop stem
G-C	33 ± 7	8.7	5.2
A-G	9 ± 1	3.1	1.2
Δ1bp	95 ± 12	9.0	4.3
In3	35 ± 3	0.2	0.5
Bulge
mBulge (AAGA/UUCU)	56 ± 4	9.0	3.5
CMV/BMV chimera
C-SLC3	21 ± 1	0.3	0.4
C-SLC5	23 ± 2	0.2	0.3

^aRNA synthesis methods were as described by Kim et al. (16) and Ranjith-Kumar et al. (24).

^bwt, wild type.

^cMutations are indicated by underlines.