TABLE 1.
Activity of CYP153A6 in cell extractsa and substrate binding parameters towards various substrates of purified protein
| Substrate | Activity (min−1)b | Solubilityc (μM) | Apparent Kd value (μM)b | High spind (%) |
|---|---|---|---|---|
| Hexane | 0.7 (0.3) | 93.4 | 3.7 (0.4) | 40 |
| Heptane | 21.2 (4.2) | 24.4 | 0.48 (0.13) | 78 |
| Octane | 60.8 (10.6) | 4.0 | 0.17 (0.03) | 87 |
| Nonane | 49.7 (11.1) | 1.22 | 0.022 (0.016) | 100 |
| Decane | 13.8 (3.0) | 0.27 | 0.010 (0.009) | 90 |
| Undecane | 0.4 (0.1) | <0.01 | 0.020 (0.003) | 38 |
| 2-Methyl octane | 35 (5) | ∼1e | 0.020 (0.008) | 95 |
| 2,5-Dimethyl hexane | ND | ∼1-2 | 1.5 (0.15) | 41 |
| Limonene | 31.2 (1.4) | 0.84 | 4.7 (0.7) | 23 |
| p-Cymene | 38.9 (7.5) | 1.49 | 5.8 (1.1) | 37 |
| Ethyl cyclohexane | ND | ∼7.8f | 4.2 (0.5) | 17 |
a
The CYP153A6 concentration in the cell extract was ∼2 μM; ND, not determined.
b
Numbers in parentheses are errors.
c
Solubility of the substrates in water at 20°C (29).
d
Percentage of high spin is given relative to nonane.
e
Estimate for 4-methyl octane from http://srdata.nist.gov/solubility/index.asp.
f
At 77°C from http://srdata.nist.gov/solubility/index.asp.