TABLE 1.
Primer sequences for the 10 loci used in MLST analysis of the chromosomes of 231 Medicago-nodulating rhizobia
| Locus and primer sequencea | Product length (bp) | Locus location |
|---|---|---|
| asd | 534 | 3617313…3618347 |
| F 5<2w9>-CGG CCG GGA GAT GCT GAA CA-3′ | ||
| R 5′-ATG CGC TTG GTG AAC TTC TTG-3′ | ||
| edD | 552 | 766634…768454 |
| F 5′-GGC ATC ATC ACC TCC TAC AA-3′ | ||
| R 5′-CGG CGT GCC GGG ATT-3′ | ||
| gap | 486 | 2975447…2976457 |
| F 5′-CGG TCC GGT CGA GAC CAA-3′ | ||
| R 5′-CGG TAG AGA TCC TTG TGC AT-3′ | ||
| glnD | 343 | 429607…432456 |
| F 5′-GTG CGC TGC CAC ATG CAY TT-3′ | ||
| R 5′-CCG GRT CRC GCT TGA A-3′ | ||
| gnd | 400 | 2091007…2092437 |
| F 5′-GGG CCG GCT CAA CTC CTA-3′ | ||
| R 5′-CGG CAT CGG CAG GTT-3′ | ||
| nuoE1 | 293 | 1381334…1382161 |
| F 5′-GCG CGC KCA GGA GCA GGA-3′ | ||
| R 5′-CGC AGG CGC CCT GAC ATT-3′ | ||
| ordL2 | 456 | 770925…772211 |
| F 5′-GCG GCG CGG TCG TCA Tx-3′ | ||
| R 5′-CGC CAT GGC CGG AAT A-3′ | ||
| recA | 357 | 1948178…1949224 |
| F 5′-CCG GTT CGC TCG GCC TCG ATA-3′ | ||
| R 5′-CGC CCA TCT CGC CCT CGA TTT-3′ | ||
| sucA | 533 | 3313082…3316078 |
| F 5′-GCT CGG CCT CGA ATA-3′ | ||
| R 5′-CCG TCA GCG ACA GGT-3′ | ||
| zwf | 535 | 769253…770728 |
| F 5′-GGG GGC ACC GGC GAT CTT G-3′ | ||
| R 5′-AGC GCA GTG CCA TCA GAT TCT-3′ |
a
F, forward; R, reverse; asd, aspartate-semialdehyde dehydrogenase; edd, phosphogluconate dehydratase; gap, glyceraldehyde 3-phosphate dehydrogenase; glnD, protein-PII uridylyltransferase; gnd, 6-phosphogluconate dehydrogenase; nuoE1, NADH dehydrogenase I chain E protein; ordL2, putative oxidoreductase protein; recA, DNA strand exchange and recombination protein; sucA, 2-oxoglutarate dehydrogenase E1; zwf, glucose-6-phosphate 1-dehydrogenase.