TABLE 1.
Strains and plasmids used in this study
| Strain or plasmida | Relevant genotype | Source or reference |
|---|---|---|
| B. subtilis strains | ||
| SMY | Prototroph | 6 |
| SMY derivatives | ||
| JE8609 | acuA::neo+ | 6 |
| JE8610 | acuC::spc+ | |
| S. enterica strains | ||
| LT2 | Wild type | Laboratory collection |
| ER2566 | Overexpression strain | New England Biolabs |
| LT2 derivative, TR6583 | metE205 ara-9 | K. Sanderson via J. Roth |
| TR6583 derivatives | ||
| JE4175 | pBAD30 | Laboratory collection |
| JE7246 | Δ1231 (acs) pta::cat+(pBAD30) | Laboratory collection |
| JE7724 | cobB1176::Tn10d(Tc) pta102::MudJ(pBAD30) | |
| JE7725 | cobB1176::Tn10d(Tc) pta102::MudJ(pCOBB8) | |
| JE7738 | Δ1231 (acs) pta::cat+(pACS7) | |
| JE7739 | Δ1231 (acs) pta::cat+(pACSA1) | |
| JE7801 | cobB1176::Tn10d(Tc)(pACUA1) | |
| JE7802 | pat1::Tn10d(Tc)(pACUA1) | |
| JE7803 | cobB1206::MudJ pat1::Tn10d(Tc)(pACUA1) | |
| JE7855 | cobB1176::Tn10d(Tc) pta102::MudJ(pACUA1) | |
| JE7856 | pat1::Tn10d(Tc) pta::cat+(pACUA1) | |
| JE8056 | cobB1176::Tn10d(Tc) pta102::MudJ(pACUC3) | |
| Plasmids | ||
| pBAD30 | Expression vector, ParaBAD bla+ | 11 |
| pACS7 | S. enterica acs+ cloned into pBAD30 | Laboratory collection |
| PACSA1 | B. subtilis acsA+ cloned into pBAD30 | |
| PCOBB8 | S. enterica cobB+ cloned into pBAD30 | Laboratory collection |
| pACUC3 | B. subtilis acuC+ cloned into pBAD30 | |
| pACUA1 | B. subtilis acuA+ cloned into pBAD30 | |
| PACSA2 | B. subtilis acsA+ cloned into pTYB12 bla+ | New England Biolabs |
| pACUA2 | B. subtilis acuA+ cloned into pTYB12 | |
| pACUC4 | B. subtilis acuC+ cloned into pTYB12 | |
| pACUA42 | B. subtilis acuA cloned into pET42a (kan+) | Novagen |
a
Unless otherwise stated, strains listed were constructed during the course of this work.