TABLE 1.
Oligonucleotides
| Primer | Oligonucleotide sequence (5′ → 3′)a | Use(s) |
|---|---|---|
| yqgG-1 | TGGAAATTCAAATTCAAGCAGC | 5′pstS probe, 5′pstS RT-PCR |
| yqgG-2 | ATGTTTATTGCTTCACTTGGTC | 3′pstS probe, 3′pstS RT-PCR |
| pstB-1 | TATTATGGAAATGTTCAGGC | 5′pstB probe |
| pstB-2 | CAATACATAATCTTTGCTGC | 3′pstB probe |
| worf-1 | AAAGAATATGATGAAAAGCTGG | 5′CAC1710 probe |
| worf-2 | TCTTCTCCACTATTTATTCCAG | 3′CAC1710 probe |
| yqgG-PE-2 | IRD800-GAACATTAACACTCACATCGGG | Primer extension and |
| yqgG-PE-3 | IRD800-ATGGTTGAAGTGCTGTAGATCC | sequencing reaction of pstS |
| vor-yqgG-Eco2 | ATTATAAAgaattcATAGCTGCTG | Cloning of pstS gene region |
| nach-yqgG-Eco3 | TCCATAGCTTgaattcAATTTAAG | into pBluescript II SK(+) |
| yqgG-a | TTAGTGAAAGTTGCTCTTGTTC | pstS cDNA |
| yqgG-3 | TTAGGTTTTGAATTTAGTTACC | 5′pstS RT-PCR |
| yqgG-4 | GTAAGTTTAGATTGTAAAGATC | 5′pstS RT-PCR |
| yqgG-8 | TACTTTGGAGGTATTTTAAATG | 5′pstS RT-PCR |
| yqgG-9 | TAACATGTTAACTGTAAAGAGG | 5′pstS RT-PCR |
| yqgG-10 | AATTAACATTAGTATAATACTC | 5′pstS RT-PCR |
| RT-ptb-a | GTCTTATACATTACATTTCCAG | ptb-cDNA |
| RT-ptb-1 | TTTGGCATTAAGAAGATATCAG | 5′ptb RT-PCR |
| RT-ptb-2 | GCATATTAAACAAAGAAGTTGG | 3′ptb RT-PCR |
a
Lowercase letters indicate nucleotide substitutions used to generate restriction sites in the PCR products.