FIG. 6.

Induction of BATF mRNA expression is B cell specific. The adherent RPMI 2650 cell line (A and C) and the HeLa cell line (B and D) were transfected as described in Materials and Methods with 5 μg of the indicated activators, 5 μg of the CBF-1 CAT reporter, and 2 μg of a β-galactosidase expression plasmid. Forty-eight hours following transfection, the cells were harvested for the preparation of RNA and protein. (A and B) RNA was analyzed by RT-PCR for BATF. As a control, the same RT samples were analyzed for the expression of β-actin mRNA by PCR. RNA from 721 B cells provided a positive control. A no-RNA (−RNA) reaction and total RNA from cells transfected with empty vector DNA served as the negative controls. ØX174 HaeIII DNA (M) was the molecular weight marker. Results indicate no activation of BATF gene expression in either cell line. (C and D) Protein extracts were normalized to β-galactosidase activity and assayed for CAT as described in Materials and Methods. The transfection was performed a minimum of three independent times to arrive at the average activities presented. Error bars indicate the standard errors of the means.