TABLE 2.
PBPs complement chaining phenotype of amiC mutants
| Straina | PBPs deletedb | PBP replacedc | Percentage of cells arranged as:
|
No. of cells counted | ||
|---|---|---|---|---|---|---|
| Single cells | Double cells | Chains | ||||
| CS109 | 41.3 ± 0.2 | 58.6 ± 0.2 | 0.00 ± 0 | 416 | ||
| RP1 | 24.1 ± 1.8 | 62.3 ± 4.3 | 13.4 ± 6.2 | 503 | ||
| RP185-1K | 5, 6, 7 | 19.0 ± 0.8 | 39.0 ± 3.0 | 41.8 ± 2.2 | 758 | |
| RP184-1K | 4, 5, 6, 7 | 10.5 ± 2.9 | 23.7 ± 2.8 | 65.7 ± 5.7 | 638 | |
| RP188 | 5, 6, 7 | 4 | 15.9 ± 8.6 | 36.2 ± 7.1 | 47.7 ± 15.9 | 437 |
| RP189 | 4, 6, 7 | 5 | 13.3 ± 5.9 | 30.8 ± 9.5 | 55.7 ± 15.7 | 786 |
| RP191 | 4, 5, 6 | 7 | 19.4 ± 1.0 | 35.3 ± 2.8 | 45.3 ± 4.2 | 535 |
| RP12 | 4, 5, 6 | 19.6 ± 0.8 | 38.5 ± 3.4 | 41.8 ± 4.3 | 1066 | |
| RP167 | 4, 6, 7 | 22.1 ± 3.7 | 44.0 ± 0.3 | 33.5 ± 3.3 | 828 | |
a
All strains contain an amiC deletion except for E. coli CS109. Strains were grown in LB medium at 37°C until the OD600 = 0.6. Data was from two independent experiments, and the mean ± standard deviation is reported.
b
Numbers refer to the respective PBPs deleted: 4, PBP 4; 5, PBP 5; 6, PBP 6; 7, PBP 7.
c
Denotes that the wild type versions of the indicated PBPs replaced genes originally deleted from the chromosome of RP184-1K.