TABLE 3.
Muropeptide profiles of amidase mutants
| Straina | Amidase(s) deleted | Percentage of muropeptideb
|
||
|---|---|---|---|---|
| Tripeptide | Tetrapeptide | Pentapeptide | ||
| CS109 | 13.5 ± 0.3 | 86.1 ± 0.4 | 0.4 ± 0.1 | |
| CS703-1 | 5.7 ± 0.6 | 60.0 ± 0.5 | 34.3 ± 0.9 | |
| RP169 | AmiA | 4.4 ± 0.3 | 55.9 ± 0.3 | 39.7 ± 0 |
| RP64-1K | AmiB | 4.6 ± 1.1 | 54.0 ± 2.2 | 41.4 ± 3.2 |
| RP19 | AmiC | 4.7 ± 0.9 | 60.1 ± 2.2 | 35.2 ± 3.1 |
| RP102 | AmiA AmiC | 4.1 ± 0.3 | 58.1 ± 0.3 | 37.9 ± 0 |
a
All strains are derived from CS703-1 (except for CS109) and carry deletions eliminating PBPs 1a, 4, 5, 6, 7, AmpC, and AmpH (4, 26). CS109 is the original parent strain.
b
Muropeptide percentages were determined by HPLC and are reported as the mean ± standard deviation of two independent measurements. Bolded results are significantly different from the respective measurements for CS703-1 (P < 0.05).