TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or description | Reference or source |
|---|---|---|
| E. coli strains | ||
| VL386 | φ(fimA-lacZ)λ pL(209)fimE::IS1 | 1 |
| VL386 recD | VL386 recD::Tn10 | 37 |
| VL386 lrp | VL386 lrp::Tn10 | This work |
| AK13 | VL386 recD::Tn10 ΔfimB::kan | This work |
| AK14 | VL386 recD::Tn10 ΔfimB::kan Lrp binding site 1 mutation | This work |
| AK15 | VL386 recD::Tn10 ΔfimB::kan Lrp binding site 2 mutation | This work |
| AK16 | VL386 recD::Tn10 ΔfimB::kan Lrp binding sites 1 and 2 mutation | This work |
| BL21(DE3) | F−dcm ompT hsdS gal | 39 |
| Plasmids | ||
| pSLD203 | fimB gene cloned in pUC18 | 13 |
| pKMC102 | lrp gene cloned in pZC320 | K. McFarland |
| pSGS501 | fimB::kan fimE::IS1 φ(fimA-lacZ) cloned in pAYC184, switch phase off | 37 |
| pSGS501Lrp-1 | Lrp binding site 1 mutated in pSGS501 | This work |
| pSGS501Lrp-2 | Lrp binding site 2 mutated in pSGS501 | This work |
| pSGS501Lrp-1/2 | Lrp binding sites 1 and 2 mutated in pSGS501 | This work |
| pKMC301 | lrp gene cloned in pET22b | K. McFarland |
| pMMC108 | fimS cloned as 550-bp fragment in the PstI site of pMMC106 | M. McCusker |
| pMMC108Lrp-1 | Lrp binding site 1 mutated in pMMC108 | This work |
| pMMC108Lrp-2 | Lrp binding site 2 mutated in pMMC108 | This work |
| pMMC108Lrp-1&2 | Lrp binding sites 1 and 2 mutated in pMMC108 | This work |
| pUC18 | Cloning vector, Apr | 45 |