FIG. 4.

Growth, DNA replication, and cell division were suppressed by overproduction of the TrfA T1 peptide but not by the TrfA T1 (ΔLF) mutant. Cells were transformed by various pBAD plasmids and grown at 37°C in LB supplemented with 100 μg/ml ampicillin and 2% arabinose. Cultures were withdrawn at the times indicated. (A) Cell growth was monitored by measuring the OD₆₀₀ of the cultures. (B) DNA replication was assessed by measuring the incorporation of [H³]dTTP into acid-insoluble materials. □, pBAD-T1; ▪, pBAD-T1 (ΔLF); •, pBAD-T2.