FIG. 1.

Representative data from the screen for vesiculation mutants. (A) Immunoblot with anti-LPS antibody of cell-free supernatants from cultures of insertion mutants. Representative high-vesiculation (circle) and low-vesiculation (square) mutant phenotypes are shown. Control wild-type (+) and washed wild-type (−) samples are at bottom right (rectangle). (B) Coomassie blue-stained SDS-PAGE of pelleted vesicles from cultures of wild-type DH5α (WT) and five Tn mutants demonstrates high vesiculation (mutants 2, 4, and 5) and low vesiculation (mutant 3) phenotypes. Molecular weight standard proteins (kDa) and the positions of Omps F, C, and A are labeled.