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Colony morphology of Gln+ transformants. Gln+ transformants were selected on glucose X-Gal minimal medium containing Casamino Acids, glutamate, and ammonium as the nitrogen sources after transformation of strain SF300G7 with mutagenized glnA DNA. (A) A blue Gln+ colony with sharp borders. (B) A blue Gln+ colony with a halo of blue Gln− cells.
