FIG. 1.

Effects of clpXP and/or grlR deletions on the expression of FliC. (A) Coomassie brilliant blue-stained SDS-PAGE profiles of secreted proteins from cells cultured in LB. An arrowhead indicates the FliC band. Strains used: wild type, SKI-5142; grlR, SKI-5152; clpXP, SKI-5149; grlR clpXP, SKI-5156. (B and C) Western blotting analyses of (B) FliC and (C) EspB in equivalent amounts of whole-cell lysates prepared from the same strains used in panel A. Cross-reacting bands recognized by the polyclonal anti-FliC (H7) serum served as loading controls.