(A and B) Repression of FliC expression by overproduced GrlA. Shown are Western blotting analyses of FliC prepared from whole-cell lysates containing an equivalent number of cells from the following strains: wild type, SKI-5142; grlR, SKI-5152; grlA, SKI-5153; grlR grlA, SKI-5154; ler, SKI-5143; and pGEM-self or pGEMGA (indicated by − or +, respectively, in panel B). (C) Western blotting analyses of FliC and EspB prepared from whole-cell lysates containing an equivalent number of cells from the same strains with plasmid: wild type, SKI-5142; ler, SKI-5143; pACYC184 or pACGA (indicated by − or +, respectively).