TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant phenotype | Source or reference |
|---|---|---|
| Strains | ||
| SKI-5142 | Wild-type EHEC O157:H7 Sakai Δ(lacZYA) | 33 |
| SKI-5143 | SKI-5142 Δ(ler)::kan | 33 |
| SKI-5149 | SKI-5142 Δ(clpXP)::kan | 33 |
| SKI-5152 | SKI-5142 Δ(grlR)::cat | 33 |
| SKI-5153 | SKI-5142 Δ(grlA)::cat | 33 |
| SKI-5154 | SKI-5142 Δ(grlR-grlA)::cat | 33 |
| SKI-5156 | SKI-5149 Δ(grlR)::cat | 33 |
| SKI-5200 | SKI-5142 Δ(fliC):: kan | This study |
| Plasmids | ||
| pGEM-T-Easy | TA cloning vector, Apr | Promega |
| pGEM-self | Self-ligated pGEM-T-Easy | 33 |
| pGEMGA | pGEM-T-Easy-grlA | 33 |
| pGEMGR | pGEM-T-Easy-grlR | This study |
| pGEMFHDC | pGEM-T-Easy-flhDC | This study |
| pACYC184 | Cloning vector; Cmr Tcr | 9 |
| pACGA | pACYC184-grlA | This study |
| pACYC177 | Cloning vector; Apr Kmr | 9 |
| pAC177FHDC | pACYC177-flhDC | This study |
| pRL124 | Promoter probe vector; Apr | 51 |
| pRLFHD | pRL124-flhD promoter region | This study |
| pRLFGA | pRL124-flgA promoter region | This study |
| pRLFLC | pRL124-fliC promoter region | This study |
| pRLLER | pRL124-ler promoter region | 33 |