FIG. 5.

Effects of temperature and osmotic pressure on Bam35-induced depolarization of HER1410 cells. After 10 min of incubation in 100 mM Tris-HCl, pH 8.0, containing 3 μM TPP⁺, the cells were infected with Bam35 using an MOI of ∼10. GD was added to the final concentration of 5 μg/ml. (A) Temperature effects (37°C, black; 32°C, green; 27°C, red; 21°C, blue). (B) Effects of osmotic pressure (95 mM sucrose, red; 190 mM sucrose, pink; 375 mM sucrose, green; 500 mM sucrose, black; 750 mM sucrose, blue). The experiments were performed at 37°C.