Map of pAPEC-O1-ColBM. The outer two circles show ORFs in forward and reverse orientation, categorized by color as follows: red, virulence associated; yellow, mobile elements; dark blue, plasmid transfer; orange, plasmid replication; olive, unknown; teal, plasmid maintenance. The third circle shows IS elements with their directions of transcription indicated by arrows. The fourth circle shows the scale in base pairs. The fifth circle shows G+C content plotted against average G+C content for the plasmid of 49.6%, with light blue indicating lower G+C values and dark blue indicating higher G+C values. The sixth circle shows the CAI (1-CAI is plotted), with pink rays indicating CAI values of <0.25 and purple rays indicating CAI values of >0.25. Maps were created using GenVision from DNAStar.