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. 2006 Aug;188(16):5831–5838. doi: 10.1128/JB.00630-06

FIG. 5.

FIG. 5.

Effects of the θ11Hot and Hot12θ chimeric proteins. The three panels show the effect of the θ11Hot and Hot12θ chimeric proteins along with the control proteins on the mutability of dnaQ49 (A), dnaQ923 (B), and dnaQ930 (C). The strains used were NR17117 (dnaQ49 ΔholE), NR16320 (dnaQ923 ΔholE), and NR16329 (dnaQ930 ΔholE) containing various plasmids as indicated along the x axis: pKO3-ΔholE, pKO3-holE, pKO3-hot, pKO3-Hot12θ, or pKO3-θ11Hot. The cultures were grown in LB with chloramphenicol at 30°C and 35°C for the dnaQ49 strains (A) and at 30°C for the dnaQ923 and dnaQ930 strains (B and C). Plates were incubated at the same temperature as the cultures. The frequencies of rifampin-resistant colonies were determined for 8 to 12 independent cultures for each strain, and the data were analyzed using Prism software (GraphPad). The graph shows the median values and interquartile ranges for the frequencies of rifampin-resistant mutants.