TABLE 2.
Oligonucleotide primers
| Primer | Sequencea |
|---|---|
| Pr1 | 5′-GAGAAATGCGGCCGCTGTAGTGTCCTTTCGTTTTATGCCC-3′ |
| Pr6 | 5′-CAAATCAGTCGACGCCAGCAGGTCGGGTTCTCC-3′ |
| hotN-lowb | 5′-CAGCTGCGCAGATTCTCTGGTTG-3′ |
| holEC-upb | 5′-CAGAGAATCTGCGCAGCTGGTTTCG-3′ |
| holEN-lowc | 5′-ATGAAATAGGTGCGCAAATGTTCAGGCTG-3′ |
| hotC-upc | 5′-TGAACATTTGCGCACCTATTTCATGGAAC-3′ |
| HotNLowd | 5′-CACTTTATCCATTTCTTCCTGACTTTTAGCTGC-3′ |
| HolECUpd | 5′-GTCAGGAAGAAATGGATAAAGTGAATGTCG-3′ |
| HolENLowe | 5′-CTTATCCCGTTCTGTTTGATCCAGTTTAGC-3′ |
| HotCUpe | 5′-GATCAAACAGAACGGGATAAGGTTAACG-3′ |
| SeqHotUp | 5′-GGAATATTGCAGCTAAAAGTC-3′ |
| SeqHotLow | 5′-CTATTTCTTTACGGCATCATC-3′ |
| SeqholE Up | 5′-CTGAAGAATCTGGCTAAACTG-3′ |
| SeqholE Low | 5′-GTTTTATTTAAGTTTGGGCTC-3′ |
a
Sequences in boldface type correspond to holE or hot coding sequences.
b
Primers with complementary 5′ ends used to create pKO3-Hot50θ.
c
Primers with complementary 5′ ends used to create pKO3-θ49Hot.
d
Primers with complementary 5′ ends used to create pKO3-Hot12θ.
e
Primers with complementary 5′ ends used in creation of pKO3-θ11Hot.