FIG. 6.

Effect of reserpine on the hemolytic activity of the wild-type strain AC475. Bacteria were grown in the presence of 0 to 25 mg/liter reserpine to late logarithmic phase and subjected to hemolysin extraction as detailed in Materials and Methods. Hemolysin extracts were incubated with a 1% erythrocyte suspension in PBS for 15 min at 37°C. To detect hemolysis, hemoglobin release was measured as the OD₅₄₀ of the supernatant. Depicted are mean values of five independent experiments.