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Norepinephrine-induced production of BfeA in B. bronchiseptica. Bacterial lysates from wild-type B013N cells grown in iron-depleted SS medium (−Fe) or in iron-depleted medium supplemented with 50 μM norepinephrine (−Fe+NE) were subjected to immunoblot analysis using a FepA-specific antiserum. The sizes of molecular mass standards (in kilodaltons) are shown on the left. Arrowhead indicates the immunoreactive BfeA protein.
