FIG. 5.

Norepinephrine-induced bfeA transcription. Strains B013N (wild type) and BRM24 (ΔbfeR) carried the bfeA-lacZ reporter plasmid pMP3 or the vector control plasmid pMP220. Strain BRM24 was complemented by using the bfeR⁺ bfeA-lacZ plasmid pMP4. Bacteria were assayed for β-galactosidase activity after growth in SS medium under the following conditions: iron replete (solid bars), iron replete and supplemented with 50 μM norepinephrine (shaded bars), iron depleted (striped bars), or iron depleted and supplemented with 50 μM norepinephrine (open bars). Error bars, ±1 standard deviation from the means of triplicate cultures.