FIG. 2.

Transduction levels of polarized airway cell lines with pseudotyped FIV vector. Primary cultures were transduced with pseudotyped FIV vector applied to the apical or basolateral surface. In addition, as a control for pseudotransduction, cells were pretreated with AZT for 24 h before vector application. Four days after initial vector incubation, cells were harvested and the β-galactosidase activity was quantified and normalized to total protein. n = 3 (samples from three independent human specimens). RLU, relative light units. ∗, P < 0.01.