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SPR experiments with the GST-TonBpp-FpvA complex. A. Control corrected sensorgrams corresponding to the successive injections of BSA (312 to 10,000 nM), FpvA (90 to 3,000 nM), and FpvA-Pvd-Fe (180 to 5,700 nM) over 80 RU of GST-TonBpp. B. Saturation curves obtained on an 80-RU GST-TonBpp surface for FpvA and FpvA-Pvd-Fe and on a 60-RU GST-TonBpp surface for FpvA-Pvd are shown.
