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. 2003 May;77(10):5975–5984. doi: 10.1128/JVI.77.10.5975-5984.2003

FIG. 5.

FIG. 5.

Western blot analysis for expression of Flag-tagged KSHV proteins in transduced ECs. (A) Expression of Flag-tagged KSHV proteins in ECs (as a control), ECs transduced with the empty LZRS retroviral vector (EC+vector), and ECs transduced with LZRS-v-cyclin (EC+v-cyclin). Note the band at approximately 30 kDa for ECs transduced with LZRS-v-cyclin. The Western blot was probed for expression of Flag to demonstrate expression of the viral protein and reprobed for actin (band at approximately 42 kDa) to demonstrate equal loading of proteins. (B) Expression of Flag-tagged KSHV proteins in ECs, ECs transduced with the empty LZRS retroviral vector (EC+vector), and ECs transduced with LZRS-v-FLIP. Note the band at approximately 22 kDa for ECs transduced with LZRS-v-FLIP. The Western blot was probed for expression of Flag and reprobed for actin. (C) Expression of Flag-tagged KSHV proteins in ECs, ECs transduced with the empty LZRS retroviral vector (EC+vector), and ECs transduced with LZRS-LANA. Note the band at approximately 240 kDa for ECs transduced with LZRS-LANA. The blot was probed for expression of LANA using a rabbit polyclonal anti-LANA antibody and reprobed for actin. A representative experiment is shown. At least three independent experiments were performed and gave similar results.

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