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Binding of CD4bs MAbs (A and C) and non-CD4bs MAbs (B and D) to gp120JR-FL and mutant GDMR in ELISA. (A and B) Antibody binding to wild-type gp120. (C and D) Antibody binding to gp120 of mutant GDMR. Supernatants containing monomeric gp120 were captured onto ELISA plate wells and probed with various concentrations of MAb, starting at 10 μg/ml. Bound antibody was detected with alkaline phosphatase-conjugated secondary antibody. Absorbance was measured at 405 nm.
