FIG. 2.

Binding of V3 loop MAbs to wild-type gp120_JR-FL and glycoprotein of mutant P313N in Western blot and in ELISA. (A) Wild-type gp120 (lane 1) and mutant glycoprotein (lane 2) were reacted with MAb b12 (1 μg/ml). (B) Glycoproteins were reacted with MAbs 19b, 447-52D, and loop 2 (pooled mixture; 1 μg of each per ml). Molecular mass indicators (bars) and the average molecular masses of wild-type and mutant glycoproteins (arrows) are shown on the left in kilodaltons. (C and D) MAb binding to wild-type gp120 (C) and mutant P313N (D) was determined by ELISA.