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Undiminished iNOS production in T. gondii-infected IGTP-deficient mice. Wild-type, IGTP-deficient (IGTP KO), and IFN-γ receptor-deficient (IFNgR KO) mice were inoculated i.p. with 20 cysts ME49 strain T. gondii. Eight days postinoculation, hepatic mRNA was prepared and used for sequential Northern blotting with iNOS and glyceraldehyde-3-phosphate dehydrogenase probes. Positions of the major ribosomal RNA species are indicated as determined from the stained gel.
