FIG. 2.

Analyses of viral RNA accumulation levels in CymRSV- and Cym19stop-infected tissues. In situ hybridization of 12 μm paraffin-embedded leaf cross sections with digoxigenin-11-UTP-labeled virus-specific RNA probe. Samples were taken from the first systemically infected leaves at 7 d.p.i. (A, D, G, and J) Near-consecutive sections of CymRSV-infected tissue. (B, E, H, and K) Near-consecutive sections of Cym19stop-infected tissue. (C, F, I, and L) Near-consecutive sections of mock-inoculated tissue. The color reaction was stopped at 20 (J, K, and L), 40 (G, H, and I), 90 (D, E, and F) and 180 (A, B, and C) min. Arrowheads in panel B indicate the sites of Cym19stop accumulation. Bar in panel A, 250 μm (applies to panels A to L).