FIG. 5.

CAT vRNA minigenome rescue by CCHF virus superinfection. Different eukaryotic cell lines were transfected with different amounts of CAT-containing CCHF virus minigenome constructs (pRF243 [pol I {m} CCHF virus S CAT {vRNA}] and pRF249 [pol I {h} CCHF virus S CAT {vRNA}]) and subsequently superinfected with CCHF virus (MOIs of 0.1 and 0.001 PFU/cell). Cells were harvested 72 h posttransfection (48 h p.i.) and analyzed for CAT activity. Lane 1, reporter gene background activity in BHK-21, HeLa, 293T, and SW13 cells upon CCHF virus infection; lane 2, reporter gene background activity after transfection of pol I vector plasmids (pRF207 and pRF240); lanes 3, 7, 11, and 15, reporter gene background activity after transfection of different CAT vRNA minigenomes (nonsuperinfected); lanes 4 to 6, CAT activity in BHK-21 cells transfected with plasmid pRF243 (Fig. 1C) and superinfected with CCHF virus; lanes 8 to 10, HeLa cells transfected with plasmid pRF249 (Fig. 1C) and superinfected with CCHF virus; lanes 12 to 14, 293T cells transfected with plasmid pRF249 and superinfected with CCHF virus; lanes 16 to 18, SW13 cells transfected with plasmid pRF249 and superinfected with CCHF virus.