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. 2003 May;77(10):5877–5888. doi: 10.1128/JVI.77.10.5877-5888.2003

FIG. 6.

FIG. 6.

Expression of PAK-T423E promotes MV replication in restrictive 3T3 cells. (A) Representative fluorescence microscopy images of nontransfected 3T3.CD4.neo cells (i, iv, and vii) and 3T3.CD4.neo cells transiently transfected with wild-type PAK-1 (PAK-WT; ii, v, and vii) or constitutively active PAK-1 (PAK-T423E; iii, vi, and ix) 24, 48, and 72 h following infection with vMyxgfp (MOI of 1). GFP-positive cells in cultures expressing PAK-T423E were more abundant and presented with larger cell bodies than those of other groups (insets). Original magnification, ×10; inset magnification, ×20. (B) PAK-T423E expression in transfected 3T3.CD4.neo cells was assessed by serial immunostaining with rhodamine red-conjugated antibody. Green and red cells indicate MV-infected (middle panel) and PAK-T423E-transfected (left panel) cells, respectively, while yellow cells (right panel) indicate concurrent infection and transfection. Original magnification, ×20.