Figure 1.

K^bm3 positively selects 2C T cells into the CD8 single-positive compartment. Flow cytometric analysis of the selection profiles for the 2C transgenic T cells in TAP-positive and -negative as well as K^bm3-positive and -negative mice is indicated. Total splenocytes or thymocytes were isolated and stained with a K^b/SIYR tetramer, as shown by the histograms. The dot plots show the CD4 CD8 profiles of the cells expressing high 2C. All dot plots are collected through region 2 (R2) of the histograms except for the TAP-positive/K^bm3-positive mice, which are collected through region 3 (R3) because of their lower 2C expression levels. The percent of gated cells that are CD8-single positive is not shown for the TAP-positive K^bm3-positive mouse, because these cells straddle the quadrant boundary.