FIG. 4.

Mapping of DNA sequence requirements for K8 binding. (A) Schematic illustration of biotinylated DNA fragments used in this assay, where the biotin label is marked with solid dots. (B) TPA-induced BCBL-1 nuclear extract was incubated with DNA fragments conjugated on magnetic beads, washed, and eluted with D500 elution buffer. Samples were assayed by Western blotting with anti-K8 antibody. (C) The nuclear extract from 293 cells transfected with pCR3.1-K8α was incubated with the DNA fragments conjugated on magnetic beads, washed, and eluted with D500 elution buffer. Samples were assayed by Western blotting with anti-K8 antibody.