FIG. 2.

Dissociation of wild-type (WT) and mutant RT complexes with template-primers. (A) Dissociation kinetics on an RNA template. (B) Dissociation kinetics on a DNA template. Equal amounts (130 nM) of RT and template-primer were preincubated for 5 min at 37°C. At time zero, preformed complexes were combined with a competing trap, and the dissociation of RT from the template-primer was monitored by measuring the incorporation of radiolabeled dNTP as described in Materials and Methods. The results presented are the means ± the standard errors of the mean from at least three separate experiments. The curves represent nonlinear least-squares fits of the data to an equation for one-phase exponential decay.