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Binding of the cyc.ext.Pep.1 to anti-MIR mAbs (a) and rat anti-AcChoR sera (b). Peptide-coated plates were incubated overnight with dilutions of indicated mAbs (a) and sera from EAMG rats, or control complete Freund's adjuvant-immunized rats (b). Bound antibody was detected with anti-rat IgG conjugated to alkaline phosphatase or with IgG2a-specific biotinylated antibodies.
