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Characterization of fusion intermediate arrested by SS-bonds. SOS and target cells arrested at the committed state were exposed to 0.5 mM CPZ either before (second bar) or after (third bar) DTT treatment. Alternatively, cells captured at the committed state and treated with DTT were allowed to bind the C34 (0.4 μM) or N36 (25 μM) peptide and then brought back to the optimal temperature (fourth and fifth bars, respectively).
