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Serum cytokine profile after inoculation with the rearranged viruses. Groups of four to five mice were inoculated intranasally with 5,000 PFU of G1N4, G3N4, N1G4(wt), or G1N2 virus per animal. Control animals were given medium only. After 4 and 7 days, serum was collected from individual animals and the levels of IFN-γ, IL-4, and IL-12 were determined. Results are expressed as the mean cytokine levels ± SEM.
