Figure 4.
Inhibition of IL-9-induced mast-cell activation and eosinophilia in immunized mice. Seven-week-old C57BL/6 mice subjected to the IL-9-OVA immunization protocol or immunized with noncoupled IL-9 and OVA or left untreated (five mice per group) were injected i.p. with 107 IL-9-producing TS1.G6 cells, 5 mo after the last boost. Five age-matched mice served as negative controls. All mice were tested 25 days later for MMCP-1 serum levels by ELISA (A), and blood leukocytes were isolated for eosinophil counting (B). Eosinophil percentages were determined by enumerating 20,000 cells per slide. Means ± SEM are indicated.