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. 2003 May;185(10):3068–3075. doi: 10.1128/JB.185.10.3068-3075.2003

FIG. 1.

FIG. 1.

Mapping of the promoter transcribing the smc gene. (A) Schematic showing the organization of the chromosomal region containing the smc gene. Bars above the line, genes (black) and an open reading frame (grey) transcribed from left to right; bars below the line, gene and open reading frame transcribed in the opposite direction. Arrow, orfA and smc promoter mapped in this study. Below is shown the extent of the regions upstream of smc that were cloned in front of a promoterless lacZ gene, resulting in different transcriptional fusions. The β-galactosidase activities (in Miller units) of strains containing these plasmids are shown. All activities reported are averages of at least four independent measurements. (B) Mapping of the transcriptional start site of the orfA and smc promoter by primer extension analysis. A DNA sequencing ladder was generated with the oligonucleotide used for the primer extension analysis. Lane 1, yeast tRNA; lane 2, total RNA isolated from Caulobacter cells. Arrow, only major band within the DNA region present in the pRBJ597 plasmid. (C) Sequence of the orfA and smc promoter region. Arrow, mapped start site; grey shading, promoter −35 and −10 elements. GAnTC methylation sites are underlined.