TABLE 2.
Bacterial strains and plasmids
| Strain or plasmid | Relevant property | Source or reference |
|---|---|---|
| E. coli strains | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]c | Stratagene |
| P4X-SB25 | met mutant thr mutant relA Hfr | R. Lavallée |
| DY330 F′ | W3110 ΔlacU169gal-490 λcI857 Δ(cro-bioA) F′ (pro-lac) | D. Bregeon |
| RDB1 | DY330 F′ ΔyggH | This study |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| Plasmids | ||
| pCR-Blunt II-TOPO | Vector for cloning of PCR fragments | Invitrogen |
| pCR-yggH | yggH ORF cloned into pCR-Blunt II-TOPO | This study |
| pCR-yggHH6 | yggHH6 ORF cloned into pCR-Blunt II-TOPO | This study |
| pET30b | Vector for recombinant protein overexpression | Novagen |
| pET-yggHH6 | yggHH6 ORF cloned into pET30b | This study |
| pYL6 |
 in vitro transcription |
This study |
| pMet(G46A) |
 (G46A) in vitro transcription |
This study |