TABLE 1.
E. coli strains used
| Strain | Relevant genotype | Source |
|---|---|---|
| AB1157 | thr-1 leuB6 Δ(gpt-proA)62 hisG4(Oc) argE3(Oc) thi-1 ara-14 lacY1 galK2(Oc) xylA5 mtl-1 rpsL31(Strr) tsx-33 supE44 rac rfbD1 mgl-51 glnV44(As) rpoS396(Am) kdgK51 | Laboratory strain |
| BW1185 | KL16 nfi-1::Cat | B. Weiss |
| BW1524 | araD139 Δ(argF-lac)U169 deoC1 flhD5301 fruA25 gyrA219 non-9 relA1 rpsL150 | B. Weiss |
| BW1525 | BW1524 moa-250::Tn10d(Tc) | B. Weiss |
| BW9113 | φ80dIIlacBK1 ΔlacMS286 argE3 hisG4 zdh-201::Tn10 thi-1 rpsE (Spcr) glnV44(As) (Sull) mtl-1 tsx-29 | B. Weiss |
| CS85 | AB1157 ruvC53 eda-51::Tn10 | M. Marinus |
| CSH106 | ara Δ(gpt-lac)5 proB F′ lacIZ proA+B+ | Cold Spring Harbor |
| JB3 | KL16 slrD300::Tn10 | KL16 × P1/MC1009 |
| JB7 | NEB51 slrD300::Tn10 | NEB51 × P1/JB3 |
| JB13.1 | NEB20 Δnfi | Link et al. (35) |
| JB13.2 | JB13.1 Δnfi (Tcs) | Spontaneous mutation |
| JB14 | JB13.2 Δnfi thiC39::Tn10 | JB13.2 × P1/NK5139 |
| JB42 | NEB55 nfi-1::Cat | NEB55 × P1/BW1185 |
| JB43 | NEB117 nfi-1::Cat | NEB117 × P1/BW1185 |
| JB44 | NEB119 nfi-1::Cat | NEB119 × P1/BW1185 |
| JB45 | NEB122 nfi-1::Cat | NEB122 × P1/BW1185 |
| JB46 | JB45 ΔrecA slrD300::Tn10 | JB45 × P1/JB7 |
| JB47 | NEB120 nfi-1::Cat | NEB120 × P1/BW1185 |
| JB48 | NEB118 nfi-1::Cat | NEB118 × P1/BW1185 |
| JC11824 | AB1157 lexA3 | M. Marinus |
| JC9239 | AB1157 recF143 | M. Marinus |
| KL16 | HfrPO45 thi-1 relA1 spoT1 | Laboratory strain |
| KM21 | AB1157 ΔrecBCD::Kan | M. Marinus |
| KW0.5 | CSH106 moeA::Kan | This study |
| KW1 | KL16 moeA::Kan | KL16 × P1/KW0.5 |
| MC1009 | araD139 Δ(araA-leu)7697 Δ(codB-lacI)3 galK16 galE15 λ− e14− recA56 srlD300::Tn10 relA1 rpsL150(Strr) spoT1 mcrB1 | R. Osuna |
| NEB1 | CSH106 rdgB3::Kan | CSH106 × P1/RPC133 |
| NEB9 | CSH106 moa-250::Tn10d(Tc) | CSH106 × P1/BW1525 |
| NEB10 | NEB9 rdgB3::Kan | NEB9 × P1/RPC133 |
| NEB18 | CSH106 nfi-1::Cat | CSH106 × P1/BW1185 |
| NEB19 | NEB9 nfi-1::Cat | NEB9 × P1/BW1185 |
| NEB20 | KL16 moa-250::Tn10d(Tcr) | KL16 × P1/BW1525 |
| NEB21 | AB1157 moa-250::Tn10d(Tcr) | AB1157 × P1/BW1525 |
| NEB32 | NEB18 rdgB3::Kan | NEB18 × P1/RPC133 |
| NEB41 | NEB19 rdgB3::Kan | NEB19 × P1/RPC133 |
| NEB51 | KL16 ΔrecA | Link et al. (35) |
| NEB55 | NEB21 priA1::Kan | NEB21 × P1/PN104 |
| NEB117 | NEB21 ΔrecBCD::Kan | NEB21 × P1/KM21 |
| NEB118 | JC9239 moa-250::Tn10d(Tcr) | JC9239 × P1/BW1525 |
| NEB119 | JC11824 moa-250::Tn10d(Tcr) | JC11824 × P1/BW1525 |
| NEB120 | CS85 moeA::Kan | CS85 × P1/KW1 |
| NEB122 | AB1157 moeA::Kan | AB1157 × P1/KW1 |
| NEB123 | BW9113 (Tcs) | Spontaneous mutation |
| NEB124 | NEB123 moa-250::Tn10d(Tcr) | NEB123 × P1/BW1525 |
| NEB125 | NEB123 rdgB3::Kan | NEB123 × P1/RPC133 |
| NEB126 | NEB124 rdgB3::Kan | NEB124 × P1/RPC133 |
| NEB127 | NEB124 nfi-1::Cat | NEB124 × P1/BW1185 |
| NEB128 | NEB126 nfi-1::Cat | NEB126 × P1/BW1185 |
| NEB129 | NEB125 nfi-1::Cat | NEB125 × P1/BW1185 |
| NEB134 | NO120 rdgB3::Kan | NO120 × P1/RPC133 |
| NEB135 | NEB134 nfi-1::Cat | NEB134 × P1/BW1185 |
| NEB136 | NO120 moa-250::Tn10d(Tcr) | NO120 × P1/BW1525 |
| NEB137 | NEB136 (Tcs) | Spontaneous mutation |
| NEB138 | NEB137 Δnfi thiC39::Tn10 | NEB137 × P1/JB14 |
| NEB139 | NEB137 rdgB3::Kan | NEB137 × P1/RPC133 |
| NEB140 | NEB138 rdgB3::Kan | NEB138 × P1/RPC133 |
| NEB152 | NEB122 ΔrecA slrD300::Tn10 | NEB122 × P1/JB7 |
| NK5139 | λ− Δ(rrnD-rrnE)1 thiC39::Tn10 | CGSC |
| NO120 | Δ(argF-lac)205 relA1 thi-1 sulA::Mu d(Ap lacX Camr) cps | Laboratory strain |
| PN104 | garB10 fhuA22 lacZ phoA4(Am) λ−ompF627(T2r) fadL701(T2r) relA1 pit-10 dinD1::Mud(Ap lac) spoT1 priA1::Kan rrnB2 mcrB1 creC510 | CGSC |
| RPC133 | AB1157 rdgB3::Kan | Laboratory strain |