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. 2006 Aug 15;4(9):e271. doi: 10.1371/journal.pbio.0040271

Figure 4. GFP:ProSAP2 Loss and Reincorporation Rates Are Accelerated by Synaptic Activity.

Figure 4

(A) A neuron (23 d in vitro) expressing GFP:ProSAP2 that was maintained for 7 d in glutamate receptor blockers (10 μM CNQX and 50 μM AP-5). Four PSDs (blue arrows at bottom of image) were photobleached and the recovery of fluorescence at these sites was monitored (B). Then, the preparation was switched to blocker-free solution and four other PSDs (red arrows at top of image) were photobleached. After collection of the first set of postphotobleach images, the preparations were stimulated at 20 Hz for 20 s every 3 min while monitoring the recovery of the photobleached PSDs (C). A comparison of mean recovery kinetics for both sets of photobleached PSDs (D) reveals that recovery kinetics were greatly accelerated by the stimulation protocol, indicating that the loss and reincorporation kinetics of ProSAP2 at individual PSDs are accelerated by synaptic activation. Note that PSD 4 from (C) was not included here as its final fluorescence exceeded its original level, indicating that the size of this PSD may have changed during this experiment. All data are shown after correcting for ongoing photobleaching as in Figure 3. Bar in (A), 20 μm.